Journal:

Article Title: Poliovirus 2A Protease Induces Apoptotic Cell Death

doi:

Figure Lengend Snippet: Poliovirus 2A protease induces cell death. (A) 293-EcR and 293-ECR-2AI2A cells were cultured for 24 h in a 96-well microtiter plate. At the indicated times, 16 wells of each cell line were treated with 2 μM MurA. MTT reagent (Boehringer Mannheim) was added to each well 2.5 h prior to viability determination, which was performed as recommended by the manufacturer. (B) 293-EcR cells (1, 2) and 293-ECR-2AI2A cells (3, 4) were cultured for 72 h in the presence of ethanol (1 and 3) or 2 μM MurA (2 and 4) and were observed by phase-contrast microscopy.

Article Snippet: MTT reagent (Boehringer Mannheim) was added to each well 2.5 h prior to viability determination, which was performed as recommended by the manufacturer. (B) 293-EcR cells ( 1 , 2 ) and 293-ECR-2AI2A cells ( 3 , 4 ) were cultured for 72 h in the presence of ethanol (1 and 3) or 2 μM MurA (2 and 4) and were observed by phase-contrast microscopy.

Techniques: Cell Culture, Microscopy