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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Exosomes Secreted from Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cells Accelerate Skin Cell Proliferation
doi: 10.3390/ijms19103119
Figure Lengend Snippet: Growth kinetics, cell cycle, and survival analyses of skin cells treated with exosomes. Exosomes collected from MSCs (MSC-exo) or iMSCs (iMSC-exo) were incubated with HaCaT (left) or HDFs (right). ( a ) Growth profile was measured in exosome-treated cells at designated study points. Negative control (NC) is cells from serum-free culture. Culture with serum (10%) was used as positive control (PC). ( b ) At 48 h of treatment, the percentage of cells in each cycle was measured by flow cytometry. Cells cultured in serum (10%) were used as positive control. ( c ) Cell proliferation analysis by MTT assay. At 48 h of exosome treatment, the absorbance of final precipitates was measured at a wavelength of 570nm, and normalized against the value obtained from serum-free negative control (NC). All data are expressed mean ± standard deviation (SD) from three replications. * p < 0.05, ** p < 0.01, and *** p < 0.005.
Article Snippet: Cells (1 × 10 4 cells/well) were overnight cultured in 96-well plates and treated with 10 μL of
Techniques: Incubation, Negative Control, Positive Control, Flow Cytometry, Cell Culture, MTT Assay, Standard Deviation